Fig. 7: SEL1LS658P disrupts its interaction with HRD1 via F668 (SEL1L)-Y30 (HRD1).
From: SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex
a Side view of SEL1L-HRD1 interaction interface to show HRD1-Y30 and SEL1L-F688 at the interface. b Side views of the aromatic-aromatic interaction between HRD1 Y30 and SEL1L F668 in SEL1L-WT (left) and S658P (right) variants. c, d Immunoprecipitation of FLAG-agarose in SEL1L−/− HEK293T cells expressing indicated SEL1L-FLAG variants to examine their interaction with HRD1 and OS9, with quantitation shown in (d) (n = 3 independent samples for each genotype). e, f Immunoprecipitation of FLAG-agarose in SEL1L−/− HEK293T cells expressing indicated SEL1L-FLAG variants to examine their interaction with HRD1 and OS9, with quantitation shown in (f) (n = 3 independent samples for each genotype). g, h Reducing and non-reducing SDS-PAGE and Western blot analyses of proAVP-G57S high molecular-weight (HMW) aggregates in WT or SEL1L−/− HEK293T cells transfected with indicated SEL1L-FLAG constructs, with quantitation of proAVP-G57S-HA HMW shown in (h) (n = 4 independent samples for each genotype). Value, mean ± SEM. n.s. not significant; Different letters (abcd) indicate significant differences at the p = 0.05 level by one-way ANOVA followed by Tukey’s post hoc test (d, f, h).
