Fig. 8: Proteomics identification of SEL1L and HRD1 interactomes.
From: SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex
a, b Immunoprecipitation of HRD1 (a) and SEL1L (b) in WT, HRD1−/− and SEL1L−/− HEK293T cells to validate the HRD1 antibody or home-made SEL1L antibody for IP (n = 3 independent repeats per group). c, d Venn diagram showing HRD1- and SEL1L- interacting proteins identified in three independent experiments of HRD1- (c) and SEL1L- (d) IP-MS. e Heatmaps of known SEL1L-HRD1 ERAD components from the IP-MS experiments. The proteins were grouped into I and II based on their dependency on SEL1L. f Scatter plot showing average log2 fold change (FC) of the PSMs in HRD1−/− cells compared to WT in SEL1L-IP-MS and of the PSMs in SEL1L−/− cells compared to WT in HRD1-IP-MS for the 16 overlapping proteins, plus DERL2. Size of the dots is proportional to the mean PSMs in WT samples. g, h Immunoprecipitation of endogenous HRD1 (g) and SEL1L (h) in WT, HRD1−/− and SEL1L−/− HEK293T cells to examine their interactions with ERAD components (two independent repeats).
