Fig. 4: The effect of temperature on LNP core structure. | Nature Communications

Fig. 4: The effect of temperature on LNP core structure.

From: Liquid crystalline inverted lipid phases encapsulating siRNA enhance lipid nanoparticle mediated transfection

Fig. 4

a–c SAXS profiles of LNP-10PE-NP1, LNP-30PE-NP1 and LNP-49PE-NP1 at 25 °C, 37 °C and 25 °C after heating (AH). d Chemical structures of the ionizable lipids DODAP and DSDAP. e Lipid compositions for the rigidification of thermolabile lamellar 10PE-LNP-NP1 to a rigid formulation 10PE-DS-LNP-NP1. f SAXS profiles of 10PE-DS-LNP-NP1 at 25 °C, 37 °C and 25 °C after heating (AH). g, h SAXS profiles 10PE-DS-LNP-NP1 and 10PE-LNP-NP1 at 25 °C, and at 1 hour intervals at 37 °C. i CryoTEM of 10PE-LNP-NP1 after incubation at 37 °C for 1 hour. White arrows indicate the formation of polymorphic and amorphous structures, black arrows indicate lamellar structure. Scale bar is 100 nm. j, k CryoTEM of 10PE-DS-LNP-NP1 at 25 °C and after incubation at 37 °C for 1 hour. Scale bars are 100 nm. Micrographs are a representative selection of a single experiment. Abbreviations used: NP = Nitrogen to phosphate ratio, h = hour. Source data are provided as a Source Data file.

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