Fig. 5: In vitro antitumor effect of ultrasmall AuPd alloy nanozymes.
From: Ultrasmall metal alloy nanozymes mimicking neutrophil enzymatic cascades for tumor catalytic therapy
Effect of (a) Au, (b) Au3Pd1, (c) Au2Pd2, (d) Au1Pd3, and (e) Pd nanozymes on CT26 cell viability (n = 3 independent experiments). f Localization of Au1Pd3 nanozymes in CT26 cells. Scale bar = 1 µm. g HClO level in CT26 cells after exposure to different concentrations of Au1Pd3 nanozymes (n = 3 independent experiments). P values are determined with one-way ANOVA Tukey’s multiple comparisons test. h Confocal microscopy images of CT26 cells after co-incubation with Au1Pd3 nanozymes and subsequently stained with 1O2 fluorescence probe SOSG. Scale bar = 100 µm. i Flow cytometry analyses of CT26 cells treated with Au1Pd3 nanozymes using SOSG and HPF as detector for 1O2 and •OH, respectively. Representative results are present (n = 3 independent experiments). All data are presented as mean ± STD. Source data are provided as a Source Data file.
