Fig. 2: Expression and enhancer association define two classes of EVT regulators. | Nature Communications

Fig. 2: Expression and enhancer association define two classes of EVT regulators.

From: The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation

Fig. 2

a Number of genes (blue), TFs (green), and SE-associated TFs (red) in class 2 and 3 genes. b Gene track view of H3K27ac signals near the candidate regulators of EVT differentiation, with red bars indicating SEs. c Expression patterns of early- and late-stage TF candidates during EVT differentiation. Values represent log2-transformed counts compared to the average. d Enriched motifs within the top 30% of H3K27ac peaks in EVT on differentiation day 3 were identified using the findMotifsGenome.pl module under HOMER (v4.11). p-values were calculated based on the default settings of the module, with a region size of 500 bp. e Western blot analysis demonstrating the expression patterns of EVT regulator candidates in CT27 TSC line. HLA-G expression was measured to validate EVT differentiation. ACTB was used as a loading control. *ZNF439 (lower band). Independent repeats, utilizing other TSC lines, were conducted as presented in Supplementary Fig. 2d. f Normalized transcripts per million value (nTPM) of EVT regulator candidates in scRNA-seq data of human first-trimester placenta (retrieved from the Human Protein Atlas19,102).

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