Fig. 5: TFAP2C primes late-stage TFs and EVT-active genes at the early-stage of differentiation. | Nature Communications

Fig. 5: TFAP2C primes late-stage TFs and EVT-active genes at the early-stage of differentiation.

From: The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation

Fig. 5

a Experimental design for time-course ChIP-seq of TFAP2C and DLX6 during EVT differentiation. b Number of TFAP2C and DLX6 ChIP-seq peaks in TSCs and differentiating cells to EVT on days 2, 5, and 8. c Enriched motifs in DLX6 ChIP-seq peaks in EVT day 5 (left) and 8 (right). Motifs were identified using the findMotifsGenome.pl module under HOMER (v4.11), and p-values were calculated using the module’s default settings. d Gene track view of TFAP2C (blue) and DLX6 (red) signals in TSCs, EVT D2, EVT D5, and EVT D8 at the locus near HLA-G. Heatmaps of (e) TFAP2C and (f) DLX6 signals on TFAP2C in TSCs unique target loci (G1), TFAP2C/DLX6-common target loci (G2), and DLX6 in EVT D8 unique target loci (G3) in TSCs, EVT D2, EVT D5, and EVT D8. PC, peak center. Heatmaps of TFAP2C in TSCs and EVT D2, and DLX6 in EVT D8 near the genes in (g) class 2 and (h) class 4. i Occupancy score of TFAP2C (left) and DLX6 (right) on DLX6 in EVT D8 unique target loci (G3) in TSCs, EVT D2, EVT D5, and EVT D8.

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