Fig. 3: EYA4 and EYA1 support PLK1 functions in centrosome biology and mitosis.

A Prophase HeLa cells with 1 or 2 centrosome foci (pericentrin staining). B Quantitation of centrosome foci number following knockdowns (n = 3, **p = 0.0061, ***p = 0.0003, ****p ≤ 0.0001, ANOVA with Tukey correction). C Integrated intensity of pericentrin foci following knockdowns (Independent cells from left to right: 103, 91, 93, 92, ***p = 0.0001, ****p ≤ 0.0001, ANOVA with Tukey correction). D PLK1 colocalization with pericentrin in G2 cells. E G2 cells identified by gating of EdU and DAPI intensities. F Quantitation of PLK1 integrated intensity at centrosomes in G2 cells (Independent cells measured from left to right: 164, 232, 22, 41, 7, 10, ***p = 0.0004, ****p ≤ 0.0001, ANOVA with Tukey correction). G Examples of spindle defects (Green: pericentrin, Red: alpha-tubulin). H Quantification of spindle defects (n ≥ 87 mitotic cells across three experiments, Independent cells measured from left to right: 92, 87, 98, 103, 127, 147, *p = 0.0238, ***p = 0.0002, siRNA experiment: ANOVA with Tukey correction, drug treatments: two-sided Student’s t-test). I Mitotic duration following knockdowns or EYA inhibition (n ≥ 90 across 3 experiments, Total number of independent cells measured from left to right: 99, 101, 100, 97, 100, *p = 0.0122, ****p ≤ 0.0001, ANOVA with Tukey correction). J Mitotic cell death following knockdowns or EYA inhibition (n ≥ 90 across 3 experiments, ***p = 0.0003, ****p ≤ 0.0001, ANOVA with Tukey correction). K Mitotic duration following overexpression of EYA4 WT or mutants (n ≥ 100 across 4 experiments, *p = 0.0373 between EV and S128D, p = 0.0274 between S128D and Ydef, ***p = 0.0003, ANOVA with Tukey correction). L Mitotic cell death following overexpression of EYA4 WT or mutants (n ≥ 100 across 4 experiments, **p = 0.0059, ANOVA with Tukey correction). All quantitative data in this figure are presented as mean values +/− SEM. Source data are provided as a Source data file.