Fig. 3: Anti-GX_P2V, SARS-CoV-2 and SARS-CoV-2 pseudovirus activity assays of eight BIAs.
From: Cepharanthine analogs mining and genomes of Stephania accelerate anti-coronavirus drug discovery
Vero E6 cells were infected with GX_P2V (MOI = 0.01) and Caco 2-N cells were infected with SARS-CoV-2 trVLP (MOI = 0.01), and both cells were treated with different concentrations of BIAs. Intracellular viral loads were detected at 48 hpi using quantitative reverse transcription–polymerase chain reaction assay and normalized with GAPDH in Vero E6 cells (A) and Caco 2-N cells (B). Cytotoxicity of these BIAs to Vero E6 cells (A) and Caco 2-N cells (B) was measured according to the CellTiter-Blue® assay. The y-axis on the left and right graphs represent mean percentage of inhibition against virus yield and cytotoxicity of BIAs, respectively. C Inhibitory analysis of SARS-CoV-2 pseudovirus infection to BHK21-ACE2 cells by different doses of BIAs. The experiment has been repeated three times with similar results. D Ranks of eight BIAs in anti-SARS-CoV-2 assays. Colors represent three assays. The data in panels A-C are presented as mean values +/− s.d. (n = 3 biologically independent samples). Source data are provided as a Source Data file.
