Fig. 1: Simultaneous single nucleus multiomic RNA-seq and snATAC-seq of adult human kidney. | Nature Communications

Fig. 1: Simultaneous single nucleus multiomic RNA-seq and snATAC-seq of adult human kidney.

From: Predicting proximal tubule failed repair drivers through regularized regression analysis of single cell multiomic sequencing

Fig. 1

a WNN UMAP plot of multiome dataset prepared from 7 samples and totaling 50,768 nuclei. PST, proximal straight tubule; PCT, proximal convoluted tubule; KIM1 + PT, KIM1-expressing injured/failed repair proximal tubule, PEC parietal epithelial cell, LH loop of Henle, DCT distal convoluted tubule, CNT connecting tubule, PC principal cell, ICA intercalated alpha, ICB intercalated beta, POD podocyte, ENDO endothelial, MES mesangial, FIB fibroblast. b Above, RNA expression of cell type markers by cell type; below, gene activity, of cell type markers by cell type. Gene activity calculated by aggregating promoter and gene body peaks in snATAC-seq dataset. c UMAP plot of aggregate snRNA-seq dataset generated from a total of 15 samples (5 from living donor biopsies from 3 individual donors and 10 from nephrectomy tissue), containing 80,634 nuclei. d Heatmap of cell type marker expression for each cell type by sample type—nephrectomy or biopsy.

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