Fig. 7: Pancreatic islet spheroids’ function enhancement through flow and vascularization.
From: A microfluidic platform integrating functional vascularized organoids-on-chip
a Generation of pre-vascularized pancreatic islet spheroids for on-chip culture within a HUVEC endothelial bed and under flow conditions (diagram created with BioRender.com). b Schematic diagram of glucose stimulation and collect of secretions performed on-chip (created with BioRender.com). c, d Comparison of the insulin secretions (c) and stimulation index = [high glucose solution]/[low glucose solution] (d) of the pancreatic islet spheroids between the various culture conditions. Each point on the graph represents the stimulation index of one pancreatic islet obtained from the measure of low and high glucose stimulation. Scale bar, 100 µm (a). Data represents mean ± s.d. Statistical significance was attributed to values of P < 0.05 as determined by unpaired t test (two-tailed). *P < 0.05 (P = 0.017 (static w/o vasc.), P = 0.029 (static with vasc.), P = 0.037 (flow without vasc.)), ***P < 0.001. Source data are provided as a Source Data file.
