Fig. 4: Regulation of MIB2 by high temperature.

a Relative MIB2 expression in the meristematic tissues of NIL-MIB2^ST082 during a transition from 22 °C to 35 °C. Seedlings were grown to the 4-leaf stage at 22 °C in moderate sunlight, transferred to 22 °C in full sunlight and incubated for 5 days, and transferred to 35 °C in full sunlight conditions. Samples were collected and analyzed at the indicated time points. Seedlings grown at 22 °C were used as a control. UBIQUITIN3 (Solyc01g056940) was used as the reference transcript. Data were compared by two-tailed Student’s t-test, **, p < 0.01; n.s. no significant difference (p > 0.05). Values are means ± SEM (n = 4 biologically independent replicates). b Immunoblot analysis of MIB2 abundance in proMIB2:MIB2-YFP-HA meristematic tissues during the transition from 22 °C to 35 °C using anti-HA (Sigma-Aldrich, H6908, 1:2000). Seedlings were grown to the 4-leaf stage at 22 °C under moderate sunlight, transferred to 22 °C under full sunlight and incubated for 5 days, and transferred to 35 °C under full sunlight conditions. Samples were collected and analyzed at the indicated time points. Seedlings grown at 22 °C were used as a control. The anti-actin (Sigma-Aldrich, A0480, 1:2000) was used as a loading control. c, d Hypocotyl lengths of MM and proMIB2: MIB2-YFP-HA complementation seedlings (c) and NIL-MIB2^ST082 and mib2cr seedlings (d) grown at 22 °C or 35 °C under a short-day photoperiod. Seedlings were grown for 7 days at 22 °C or for 3 days at 22 °C, transferred to 35 °C, and grown for an additional 4 days before being photographed. Representative images of seedlings (left). and quantification of hypocotyl length (right). Scale bars, 1 cm. Data were compared by two-tailed Student’s t-test, **, p < 0.01; n.s. no significant difference (p > 0.05). Values are means ± SEM. n in (c, d) = biologically independent samples. Source data are provided as a Source Data file.