Fig. 4: Cbp1 enhances CRISPR array transcription from leader promoters in a minimal transcription system. | Nature Communications

Fig. 4: Cbp1 enhances CRISPR array transcription from leader promoters in a minimal transcription system.

From: Cbp1 and Cren7 form chromatin-like structures that ensure efficient transcription of long CRISPR arrays

Fig. 4

Synchronised reconstituted in vitro transcription experiments with the strong T6 promoter fused to the first ~500 bp of S. solfataricus P2 CRISPR array B (a), the same CRISPR array sequence inverted (b), and the same sequence with the first CRISPR repeat randomised (c) in the presence or absence of 300 nM Cbp1. As control for a transcription template lacking Cbp1 binding sites we used a fusion of the T6 promoter to the rpo5 gene from the archaeon Methanocaldococcus jannaschii68 (d). Time points 1, 2, 3, and 4 min after release of RNAP from the promoter are shown. Lane profiles for time point 3 min with Cbp1 (red) or without (blue) are depicted on the right of each gel. Representative gels from three replicate experiments are shown.

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