Fig. 6: Colocalization of SG markers with RNA-binding proteins.

hOLs were exposed to SA or NG conditions for the indicated periods of time and stained with antibodies recognizing G3BP1 (SG protein component) and RBPs, including PABP, hnRNP A1, and TDP-43. A Representative confocal images showing colocalization of PABP with G3BP1 in cells exposed to SA or NG conditions. B Representative confocal images showing mislocalization (and some decreased expression) of hnRNP A1 in hOLs and some colocalization with G3BP1⁺ granules at 4 h under NG conditions. C Representative confocal images showing mislocalization (and some decreased expression) of TDP-43 in hOLs but no colocalization with G3BP1⁺ granules at 4 h under NG conditions. Treatment with SA resulted in mislocalization (and some decreased expression) of hnRNP A1 (B) and TDP-43 (C) with the formation of aggregates independent of G3BP1⁺ SGs (arrowheads). Scale bars, 10 µm. Merge pictures display DAPI (blue), O4 (gray), G3BP1 (green) and the marker of interest (red). G3BP1 was used as the marker of reference for SG formation. Arrows indicate colocalization between G3BP1 and the RBP of interest. For each RBP, the colocalization experiments have been repeated 3 times in biologically independent samples, showing similar results. Ctrl optimal media, SA sodium arsenite, NG no glucose.