Fig. 7: ScRNA sequencing analysis of SG-associated genes in OL population in MS patients.

These analyses were performed on OL cell population exclusively using the SG-associated gene list recently established by the Wang group (26). A–C Molecular signature of OL cell population from publicly available datasets initially generated by the Schirmer group, (12) the Jäkel group, (25) and the Absinta group. (11) Single sample gene set enrichment analysis (ssGSEA) showing OLs from Schirmer (A) dataset (Ctrl, n = 8; MS, n = 11), Jäkel (B) dataset (Ctrl, n = 5; MS, n = 3) and Absinta (C) dataset (Ctrl, n = 3; MS, n = 5). D Euler Venn diagram of the significantly upregulated genes in MS patients compared to controls. The analysis was performed on the chronic active and chronic inactive lesions of the Absinta dataset. E Bubble plot of logFC depicting the 32 shared SG-related genes between the chronic active and chronic inactive lesions from (D). The color scale indicates logFC expression as compared to control groups; the size of the bubble denotes adjusted p value of genes expressed in MS compared to control groups. The periplaque area is added here as a comparator. F, G Bulk RNA sequencing analysis of SG-related genes in hOLs after 2 days of treatment of TNFα (n = 3), IFNγ (n = 2) or NG (n = 3) conditions using the list established by the Wang group. (26) The ssGSEA analysis compares the molecular signature of hOLs exposed to the corresponding treatments (F). The heat map shows the logFC of significant upregulated genes in NG conditions amongst the different treatments (G). Columns indicate individual samples grouped by treatment; the color scale indicates logFC expression as compared to control groups. Each dot in the graphs corresponds to an independent biological replicate. All data are expressed as mean values ± SEM, analyzed by unpaired two-tailed Student’s t test. All significant P values are indicated; ns or unlabeled not significant. Source data are provided as a Source Data file.