Fig. 5: Pancreas exosomes-mediated inter-organ communication increases throughout PDAC progression.

a Dot plot representing the average radiant efficiency fluorescence levels of CD63-mCherry⁺ Exos in the different organs in health (Panc-CD63-mCherry, n = 6) and in PDAC (KPF CD63-mCherry, n = 4). Two-tailed Mann Whitney, p = 0.0095. b Fold change of the average radiant efficiency fluorescence levels of CD63-mCherry⁺ Exos in the different organs in PDAC (KPF CD63-mCherry, n = 4) in relation to the healthy context (Panc-CD63-mCherry, n = 6). Two-tailed Mann-Whitney test, *p = 0.0119, **p = 0.0095. c Dot plot representing the average radiant efficiency fluorescence levels of PDAC CD63-mCherry⁺ Exos across different organs in KPF CD63-mCherry mice (n = 4) at time of euthanasia (left), with representative IVIS images (535 excitation laser and DsRed emission filter; right). d Dot plot representing the average radiant efficiency fluorescence levels of pancreas CD63⁺ Exos across all organs in different disease stages, healthy (Panc-CD63-mCherry, n = 6), early PDAC (KPF CD63-mCherry, n = 1 and KPC-ExoBow, n = 2) and late PDAC (KPF CD63-mCherry, n = 4). Two-tailed Mann-Whitney test, in healthy vs. early PDAC: lung p = 0.0119, kidneys p = 0.0238, heart p = 0.0238 and healthy vs. late PDAC: lung p = 0.0119, kidneys p = 0.0095. e Dot plot representing the average radiant efficiency fluorescence levels of CD63-XFP⁺ Exos present in the different organs in health (Panc-CD63-mCherry, n = 6) and in early PDAC (KPF CD63-mCherry, n = 1 and KPC-ExoBow, n = 2). Two-tailed Mann–Whitney test, p = 0.0476. f Representative confocal microscopy images of PDAC CD63⁺ Exos (green) accumulation in the lungs and kidneys of KPF CD63-mCherry mice at early (upper panel) or late (lower panel) PDAC stages. Nuclei were counterstained with hoechst (blue). Scale bar 20 μm. Data are Mean ± SEM. ING LN, inguinal lymph nodes, AXL LN axillary lymph nodes, MST LN mesenteric lymph nodes. Source data are provided as a Source Data file.