Fig. 4: The mECM@IL4 + PM@IGF1 composites induce the polarization of the pro-regenerative macrophage phenotype. | Nature Communications

Fig. 4: The mECM@IL4 + PM@IGF1 composites induce the polarization of the pro-regenerative macrophage phenotype.

From: Elastic porous microspheres/extracellular matrix hydrogel injectable composites releasing dual bio-factors enable tissue regeneration

Fig. 4

A Diagram of bone marrow-derived macrophages (BMDMs) extraction and coculture with composites. B Fluorescence images of CD68 (green) and CD206 (red) after different treatments. White arrows: morphologically extended macrophages; nuclei: blue. C Corresponding statistical analysis of CD206 fluorescence intensity. The error bars represent the minimum and maximum values; the line inside the box represents the median; box limits represent the 25th and 75th percentiles (n = 6 biologically independent samples). **P < 0.01, *P < 0.05, ns: no significant difference, one-way ANOVA, multiple comparisons. D, E Schematic diagram of the morphological change of BMDMs and corresponding statistics. Data are presented as mean ± SD (n = 25 biologically independent cells). F Flow cytometric analysis of BMDMs showing the expression of CD206+ gated cells after different treatments for 3 days. G, H Histogram of the peak shift of CD206 expression and corresponding statistical results. Data are presented as mean ± SD (n = 3 biologically independent samples). ****P < 0.0001, ***P < 0.001, **P < 0.01, one-way ANOVA, multiple comparisons. I–N Luminex microarrays detecting the inflammation-related factors secreted by BMDMs after different treatments on day 1 and day 3. Data are presented as mean ± SD (n = 3 biologically independent samples). ***P < 0.001, **P < 0.01, *P < 0.05, ns: no significant difference, one-way ANOVA, multiple comparisons.

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