Fig. 1: Fibroblasts of the big-footed bat (Myotis pilosus; MPI for short) are resistant to malignant transformation.
From: Experimental evidence for cancer resistance in a bat species
a Immunoblotting shows no significant differences in the expression of oncogenic HRAS(G12V) and SV40 LT among fibroblasts from seven bat species, as well as mouse. The experiment was repeated independently at least three times with similar results. b Anchorage-independent growth assay of the fibroblasts with and without (control) expressing HRAS(G12V) and SV40 LT for seven bat species and mouse. MPI fibroblasts expressing HRAS(G12V) and SV40LT (MPI-SFHRAS\SV40LT) form remarkably smaller colonies compared to the other bat species and the control. The representative microphotographs of the colonies grown in soft agar after 4 weeks are shown at 10× magnification, with a scale bar of 100 μm. c Quantitative analysis reveals that the area of the colonies formed by MPI-SFHRAS\SV40LT is significantly smaller than those from the other species. The n values represent the number of colonies from three independent experiments. All data are presented as mean ± SD. The P values are from two-tailed Student’s t tests. ****P < 0.0001. Source data are provided as a Source Data file.
