Fig. 4: Description and growth measurement of Chlamydomonas HCS mutants generated by CRISPR-Cas9.
From: Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida
a Schematic of the CreHCS1 target site (red arrow) and the mutated region (shown in red) in mutant HCS1-9 (1 bp deletion) and HCS1-25 (24 bp insertion). b Schematic of the CreHCS2 target site (red arrow) and the insertions (the number of inserted nucleotides was labeled in red) of mutant HCS2-27, HCS2-22, and HCS2-35. c Growth curve of g1 and HCS mutant strains cultivated in TAP medium under light and dark conditions. Values shown are the average of three biological replicates ± SD. Pictures are representative of strains on day 3 from three experimental replicates. Source data are provided as a Source Data file.
