Fig. 4: Pharmacological stimulation of collagen synthesis is positively associated with collagen uptake and MRC2 expression.
From: Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover
Flow-cytometry based collagen-uptake assay in U937 (a) or MRC5 (b) cells after drug treatment as indicated vs. vehicle control. N = 4 per group; p = 0.0205 (a), p = 0.0138 (b). Western blot of corresponding cells and conditions shown below, each representative of N = 3-4 independent experiments. Q-RT-PCR in MRC5 cells after drug treatment as indicated vs. vehicle control. N = 5 (c), 4 (d), 3 (e) independent experiments; p = 0.0043 (c), p = 0.0102 (d), p = 0.0048 (e). Western blot of corresponding cells and conditions shown below, each representative of N = 3 independent experiments. Data are shown as the mean ± SEM. Statistics: a–e unpaired Student’s t test (two-sided). *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.
