Fig. 9: Collagen biosynthesis and MRC2 expression are positively correlated in lung tissue, but this relationship is impaired in human fibrotic lung disease.
From: Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover
a UMAP plot with cell type annotation of scRNAseq data from multiple available datasets of human lung control and IPF tissue (GSE136831, GSE135893, GSE121611, GSE128033, GSE132771). N = 184,672 single cells. b, c Gene expression of individual genes as indicated in UMAP as generated in panel a. d Comparison of gene expression in all cells from data as in panel a that had non-zero expression of COL1A1 or MRC2; p < 0.0001. e Comparison of gene expression in whole human lung microarray data from GSE110147 analyzed via GEO2R in pulmonary fibrosis lungs vs. control specimens. N = 37 vs. 11 per group; p < 0.0001. f Comparison of microarray gene expression in human lung bronchoalveolar lavage specimens from GSE70867 analyzed via GEO2R from Idiopathic Pulmonary Fibrosis (IPF) lungs vs. control specimens. N = 112 vs. 20; p = 0.0016. g Comparison of microarray gene expression in peripheral blood mononuclear cell (PBMC) specimens from GSE37858 analyzed via GEO2R from Idiopathic Pulmonary Fibrosis (IPF) lungs vs. control specimens. N = 70 vs. 35; p = 0.0083. h Comparison of gene expression in whole mouse lung microarray data from GSE40151 analyzed via GEO2R from Bleomycin-treated mice vs. Saline control mice. N = 56 vs. 55; p = 0.9189. Statistics: ANCOVA of two groups (no multiple comparisons correction), p-values indicated in figures. Pearson correlations are displayed in panel d. Source data are provided as a Source Data file.
