Fig. 7: HSPG-containing gelatin scaffold facilitates neuronal regeneration with functional recovery after cortical injury.
From: Identification of the growth cone as a probe and driver of neuronal migration in the injured brain
a Experimental design for assessment of neuronal maturation and gait behavior. b–e Z-stack projection images of coronal brain sections show that EmGFP-labeled (green) V-SVZ-derived cells in the vicinity of the injury express mature neuronal marker NeuN (white). Magnified views of the EmGFP+NeuN+ cells in the boxed areas are presented in (d and e) (white arrowheads). f Quantification of EmGFP+NeuN+ cells in injured cortex. The graph shows the total number of EmGFP-labelled NeuN+ cells in P30 control or Sdc2-containing gelatin fabric-implanted injured cortices. A dot represents the examined brain. g Foot-fault test in mice with or without cortical injury and gelatin fabric implant. The graph represents the frequency of left hindlimb fault-steps. h Experimental design for the experiment using NSE-DTA mice. i Foot-fault test on adenovirus expressing Cre recombinase-injected NSE-DTA mice and wild-type mice, implanted with Sdc2-including gelatin fabric. The graph represents the frequency of left hindlimb fault-steps. Scale bars: b, c, 100 µm; d, e, 10 µm. *p < 0.05, **p < 0.01, ***p < 0.005. Error bars indicate mean ± SEM.
