Fig. 4: Further validation and application of the NH2OH and HNO products of N2 disproportionation with (H2O)2+•.
From: Efficient catalyst-free N2 fixation by water radical cations under ambient conditions
a Electrolytic reduction of the collected NH2OH product (corresponding signal at m/z 34) into NH3 (corresponding signal at m/z 35) confirmed by mass spectrometry detection. The signals at m/z 36 and m/z 37 correspond to (H2O)2+• and H+(H2O)2. b The kinetics of electrolytic reduction of NH2OH collected at different discharge voltages into NH3 determined using the indophenol blue method42 (see Supplementary Fig. 9). c The conversion of cysteine (corresponding signal at m/z 122) into cystine (corresponding signal at m/z 241) via the reaction of cysteine with the collected HNO product of the disproportionation reaction of N2 confirmed by mass spectrometry detection. d The kinetics of cysteine conversion into cystine via the reaction with the collected HNO product (~25 μM; collection time 30 min) at different cysteine concentrations. e Effects of different concentration levels of HNO alone on HT22 cell reducing capacity. f HT22 cells were pretreated with different concentration levels of HNO for 24 h and incubated with or without H2O2 (200 μM) for 1 h. Cell reducing capacity as determined with Cell Counting Kit-8 assay. *P < 0.05 and **P < 0.01versus control, #P < 0.05 and ##P < 0.01 versus model. The error bars indicate the standard deviation (n = 3).
