Fig. 3: EB, EO, and PT inhibit the conidial germination of B. cinerea and M. oryzae in a dose-dependent manner.

a Morphological differences of germinating conidia of B. cinerea in the presence of different concentrations of autophagy inhibitors EB, EO and PT at 6 hpi. Dead conidia are often observed at 15 µM and no germination is observed at 50 µM of all compounds. n = 3 biologically independent experiments. A representative image is shown. Scale bars, 20 µm. b Germination percentages of B. cinerea in the presence of indicated concentrations of autophagy inhibitors EB, EO and PT, compared to the mock control at different time points. All chemicals tested significantly reduce germination at concentrations above 15 µM. The statistical analyses are summarized in Supplementary Table 1. n = 3 biologically independent experiments. c Suppression of conidial germination and appressorium formation of M. oryzae under EB treatment. Photographs were taken at 4 and 8 hpi. Yellow arrowheads and red arrows indicate abnormal hyphae and dead conidia, respectively. The experiments were repeated three times and similar results were observed. A representative image is shown. Scale bars, 50 μm. d The delay in germination of M. oryzae is observed at low concentrations of EB and the germination rate is significantly reduced above 7 µM EB. The statistical analyses are summarized in Supplementary Table 2. n = 3 biologically independent experiments. e Significant suppression of appressorium formation of M. oryzae is observed above 10 µM EB. All graphs display means with SE. Two-way ANOVA test with Dunnett’s multiple comparison. A different number of asterisks indicates statistically significant differences. n = 3 biologically independent experiments. Summary of the statistical analyses is in Supplementary Table 3.