Fig. 1: Tab provides anti-phage defence.
From: Anti-phage defence through inhibition of virion assembly
a Phage infection assays. Ten-fold serial dilutions of indicated phages on PA14(ΔCRISPR) and PA14(ΔCRISPR) expressing Tab. Images shown are representative of three biological replicates. b Gene diagrams of the gene 31 (tab) region in related phages. The small and large terminase genes encode proteins that share >90% amino acid sequence identity among these phages. The arrow indicates the promoter of gene 31. Scale bar, 500 base pairs. c Southern blot analysis assessing the replication and accumulation of DMS3 genomic DNA during phage infection in the absence and presence of Tab. DMS3 genomic DNA was detected using digoxigenin-labeled ssDNA probes. RpoD was used as a loading control. The data shown are representative of three biological replicates. Source data are provided. d Southern blot analysis assessing the accumulation of packaged DMS3 genomes during phage infection in the presence and absence of Tab. Purified DMS3 genomic DNA and mature DMS3 phage particles were used as controls (right). Time P.I., time post-infection. The data shown are representative of three biological replicates. Full-sized blots are presented in Supplementary Fig. 1a.
