Fig. 3: Tab targets the tape measure protein (TMP).
From: Anti-phage defence through inhibition of virion assembly
a Diagram showing tail gene operon synteny and corresponding protein sequence conservation between phages sensitive or insensitive to Tab activity. The gene product (Gp) labels correspond to the gene number in phage DMS3. Genes encoding proteins sharing >30% amino acid sequence identity are colored the same. The numbers between phage operons indicate the percent shared protein sequence identity. Scale bar, 1000 base pairs. b A matrix highlighting the percent amino acid sequence identity shared between the tail proteins of phage DMS3 and the phages indicated in the left column. Phages DMS3, JBD26 (in the absence of anti-Tab), D3112 MP29 and JBD5 are sensitive to Tab activity, whereas JBD16C, JBD88a, and MP22 are insensitive to Tab-mediated defense. c Ten-fold serial dilutions of the noted phages plated on cells in the absence (left) and presence (right) of Tab. Black arrow indicates very small plaques. Image with black dashed border is zoomed in to see very small plaques. Data are representative of three independent biological replicates.
