Fig. 5: Adipocyte Ets1 knock-out mice are resistant to HFD-induced obesity.
From: M2 macrophages independently promote beige adipogenesis via blocking adipocyte Ets1
Ets1 f/f (Flox) and adipocyte-specific knock-out (EAKO) mice at the age of 4-5 weeks were fed a high-fat diet (HFD) for 14 weeks. A Body weight curves during HFD feeding (n = 6 per group). B Adipose tissue weight (n = 6 per group). C General morphology of the mouse bodies and fat pads. Red arrow, BAT; white arrow, iWAT; blue arrow, eWAT. D H&E staining of iWAT and BAT sections. Scale bar: 200 μm. E The random and fasting (14 h) blood glucose levels (n = 6 for random, n = 7 for fasting). F Glucose tolerance test (GTT) assay (n = 7 per group). G Calculated AUC of F. H Insulin tolerance test (ITT) assay (n = 7 per group). I Calculated AUC of H. J–M After 14 weeks of HFD feeding, the metabolic parameters of Flox and EAKO mice were analyzed using a home-cage system. The black and white bars represent night and day, respectively, and the arrow areas were calculated. J Daily food intake (n = 5 per group). K Daily locomotor activity (n = 6 for Flox, n = 5 for EAKO). L Oxygen consumption level (n = 6 for Flox, n = 5 for EAKO). M The calculated AUC of L. Data are means ± SEM. Two-sided Student’s t-test was used to evaluate statistical significance. Source data are provided as a Source Data file.
