Fig. 7: In-depth characterization of the LDTg, VTg, DTg, and Sph.
From: A spatially-resolved transcriptional atlas of the murine dorsal pons at single-cell resolution
a Overlay of Franklin-Paxinos atlas anatomic boundaries on MERFISH image depicting Slc32a1, Chat, and Tnc transcripts. (scale bar: 250 μm). b Donut plots: the inner plot shows the overall contribution (%) of each cluster to the total LDTg/LDTgV neurons; the outer plot classifies the clusters as glutamatergic (red), GABAergic (light blue) and cholinergic (yellow). c Stacked area charts of the LDTg/ LDTgV cluster trajectory. Clusters are color-coded according to the legend in (b). d Left: donut plot showing the LDTg cell partition in glutamatergic (red), GABAergic (blue), and cholinergic (yellow) in this study and as reported by Luquin et al. Right: estimation of Glp1r+/Slc32a1+ and Glp1r+/Slc17a6+ cells in mouse LDTg by this study and as reported by Hernandez et al. e Schematic from the Paxinos atlas showing the VTg anatomical location. f MERFISH image showing cluster at4_6 (VTg neurons; cyan polygons) along with Slc32a1, Slc17a6, and Tph2 transcripts. (scale bar: 100 μm). g Donut plots: the inner plot shows the overall contribution (%) of each cluster to the total DTg neurons; the outer plot classifies the clusters as glutamatergic (red) and GABAergic (light blue). h Schematic from the Franklin-Paxinos atlas showing the DTg, Sph, NI, CGA, and CGB anatomical location from −5.02 to −5.8 bregma level. For (e, h), abbreviations refer to Table 1. i Stacked area charts of the DTg cluster trajectory. Clusters are color-coded according to the legend in (g). j Overlay of Franklin-Paxinos atlas anatomic boundaries on MERFISH images depicting Slc32a1, Ebf2, and Rfxfp1 (top) and Ebf2 and Rfxp1 marker genes (bottom) in the Sph. (scale bar: 100 μm). k Overlay of Franklin-Paxinos atlas anatomic boundaries on MERFISH images depicting Slc32a1 and Slc17a6 (top) and 9 marker genes (bottom) in the DTg across the same rostrocaudal levels. (scale bar: 100 μm). Legend is on the right side of both panels. In (b, g), only clusters contributing >0.5 % to the overall neuronal population were plotted.
