Fig. 2: Single cell RNA profiling of newly diagnosed AML bone marrows. | Nature Communications

Fig. 2: Single cell RNA profiling of newly diagnosed AML bone marrows.

From: Comprehensive characterization of IFNγ signaling in acute myeloid leukemia reveals prognostic and therapeutic strategies

Fig. 2

A OncoPrint of patients included in the single cell RNA (scRNA) profiling. B UMAP projection of 107,067 cells passing quality control (see Supplementary Fig. 2 for QC) and cell cluster identities. C Marker gene expression for key marker genes defining cell cluster identities. D Relative proportions of cells by patients. E Example of concordance between inferCNV results and the karyotype of a patient (PT28A), with a loss of chromosome 7 shown in G-banding karyotype and loss of transcripts corresponding to chromosome 7 in inferCNV map. F Example of concordance between gene expression of monocytic AML-defining markers determined by scRNA profiling and protein expression determined by flow cytometry (CD34 negative, CD33 positive, and CD64 positive) in a representative patient with monocytic AML (PT32A). G Concordance of AML blast count by flow cytometry and scRNA profiling for all patients profiled. T test was used to evaluate the significance of Pearson correlation. Source data are provided as a Source Data file.

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