Fig. 2: Similar distribution of CD4 T-cell subsets in the SLOs of old WT and young adult Foxo1^TKO mice.

a Foxp3/CD44 representative dot-plots are shown for spleen CD4 T cells from the indicated representative mice. b Distribution of CD4_N, CD4_M, and CD4_R cells among CD4 T cells in the indicated SLOs (pLNs (peripheral Lymph Nodes), mLNs (mesenteric Lymph Nodes) and Spleen) of young adult and old WT mice versus young adult Foxo1^Ctrl and Foxo1^TKO mice. c Total cell numbers of CD4_N, CD4_M and CD4_R cells recovered from the SLOs of the indicated mice. d Absolute cell numbers of total, double-positive (CD4⁺CD8⁺), triple-negative (CD4^- CD8^- TCR_β^-), CD4_N and CD8_N thymocytes from 1-month-old Foxo1^Ctrl and Foxo1^TKO mice. e 1-month-old Foxo1^Ctrl and Foxo1^TKO mice were injected intrathymically with FITC and sacrificed 16 h later. FITC/FSC representative dot-plots are shown for CD4 T cells from the spleen, lymph nodes, and the thymus of representative mice (left panel). Absolute cell numbers of FITC⁺ CD4_N and CD8_N cells from the spleen and lymph nodes (right panel). f–h 3.10⁶ CD4_N cells from the thymus of 1-month-old CD45.1 Foxo1^TKO or Foxo1^Ctrl mice were injected i.v. into 1-month-old CD45.2 Foxo1^TKO or Foxo1^Ctrl mice respectively. Diagram illustrating the experimental model (f). Distribution of CD4_N, CD4_M, and CD4_R cells among donor CD4 T cells recovered from the indicated SLOs of recipient mice (g). Absolute cell numbers of CD4_N, CD4_M, and CD4_R donor cells recovered from the indicated SLOs of recipient mice per 10⁶ injected cells (h). Quantifications are represented as Means ± SEM. The significance of differences between two series of results was assessed using Student’s unpaired t test (c–e, h). Significant (p < 0.05) or almost significant (0.05 < p < 0.10) p-values are indicated. Source data are provided as a Source Data file.