Fig. 3: Memory and regulatory T cells from the SLOs of old WT and young adult Foxo1^TKO mice exhibit an exhausted phenotype.

a p value/Z score dot-plots representing the canonical pathways determined by Ingenuity Pathway Analysis software as significantly (p < 0.05) involved in the transcriptomic signature of CD4_N cells from old WT (left panel) or young adult Foxo1^TKO (right panel) mice. b A list of genes up-or down-regulated in CD4 T cells undergoing exhaustion in the course of a chronic infection was determined (GSE30431 Day 30). Expression of these genes by CD4 T_N cells from old WT (upper panel) or young adult Foxo1^TKO (lower panel) mice was visualized using the GSEA software. c PD1 fluorescence histograms of the indicated T-cell subsets from the spleen of a representative old mouse and a representative young adult mouse are shown (upper panel). Percentages of PD1⁺ cells among CD4_M, CD4_R, and CD8_M cells are shown for the indicated SLOs of old/young adult mice (lower left panel) and Foxo1^TKO/Foxo1^Ctrl mice (lower right panel). d Same as in (c) for KI67. e Percentages of PD1⁺ cells among CD4_M, CD4_R and CD8_M cells from the spleen of old mice are shown as a function of their Foxo1 relative expression. f Percentages of PD1⁺ cells among CD4_M, CD4_R and CD8_M cells from the spleen of old mice are shown as a function of their expression of KI67. Quantifications are represented as Means ± SEM. The significance of differences between two series of results was assessed using Student’s unpaired t test (a, c, d). The p-values for the GSEA test statistics are calculated by permutation (b). For assessing correlations, Pearson correlation (two-sided test, coefficient and 95% confidence intervals) was used (e, f). Significant (p < 0.05) or almost significant (0.05 < p < 0.10) p-values are indicated. Source data are provided as a Source Data file.