Fig. 7: Type 1 interferons induce Foxo1 down-regulation in T cells.

a Purified T cells from young WT mice were cultured for 4 days with IL-7 alone or together with the indicated inhibitors in the presence or absence of IFNα4. Diagram illustrating the experimental model. b Relative Foxo1 MFIs for CD4_N, CD4_M and CD4_R cells recovered after 4 days of culture with IL-7 in the presence of IFNα4 and the indicated inhibitors. Relative MFIs were calculated after barcoding by dividing the MFI of a given T-cell subset in the presence of one given inhibitor and IFNα4 by the MFI of the same T-cell subset cultured with the same inhibitor alone. Statistics were calculated to compare Foxo1 down-regulation induced by IFNα4 alone with Foxo1 down-regulation induced by IFNα4 in the presence of the indicated inhibitors. c–e CTV-labeled purified CD4_N cells from WT mice were precultured or not with IFN-α and then stimulated for 4 days with anti-CD3 in the presence of splenocytes from CD3_ε^KO mice. Diagram illustrating the experimental model (c). Percentages of PD1⁺ cells and PD1 MFI among the progeny of CD4_N cells upon in vitro activation (d, n = 6 independent experiments). The average number of cell cycles undergone by CD4_N cells upon in vitro activation was calculated and plotted (e, n = 6 independent experiments). Each pair of dots represents an individual experiment. Quantifications are represented as Means ± SEM. The significance of differences between two series of results was assessed using Student’s unpaired (b) or paired (d, e) t test. Significant (p < 0.05) p-values are indicated. Source data are provided as a Source Data file.