Fig. 8: Analysis of the T-cell compartment of old Ifnar^KO mice.

a Cell suspensions from the spleen of a 3-month-old WT mouse (Young adult), a 22-month-old WT mouse (Old) and a 22-month-old Ifnar^KO mouse (old) were first stained separately with anti-CD45 antibodies conjugated to different fluorochromes and then mixed before further staining. Relative Foxo1 MFIs were calculated by dividing the MFI of a given T-cell subset of old mice by the MFI of the same T-cell subset of the barcoded young adult WT mouse. b Relative expression of CCR7 and CD62L by CD4_N and CD8_N cells from the spleen of old mice as a function of their Foxo1 relative expression. Relative MFIs were calculated by dividing the MFI of a given T-cell subset of old mice by the MFI of the same T-cell subset of the barcoded young adult WT mouse. c Distribution of CD4_N, CD4_M and CD4_R cells among CD4 T cells and of CD8_N, CD8_M, and CD8_R cells among CD8 T cells recovered from the indicated SLOs of old WT versus old Ifnar^KO mice. Percentages of PD1⁺ (d) and KI67⁺ (e) cells among CD4_M, CD4_R and CD8_M cells recovered from the indicated SLOs of 22-month-old WT versus 22-month-old Ifnar^KO mice. Quantifications are represented as Means ± SEM. The significance of differences between two series of results was assessed using Student’s unpaired t test (a, d, e). For assessing correlations, Pearson correlation (two-sided test, coefficient and 95% confidence intervals) was used (b). Significant (p < 0.05) or almost significant (0.05 < p < 0.10) p-values are indicated. Source data are provided as a Source Data file.