Fig. 2: Optimization of virus stock production.
a Comparison of the susceptibility of different insect cell lines for recombinant baculovirus (rBV) infection. Three different rBVs were titrated on High Five cells as well as on two Sf9 cell lines obtained from different sources. The averaged TCID50-values of four independent experiments are presented as mean values ± SD. High Five cells exhibit the highest apparent titer due to their increased susceptibility. b Comparison of virus production of High Five and Sf9 cell lines. Two different rBVs (with/ without crystal production capability) were amplified on the denoted cell lines in three independent experiments and titrated twice on High Five cells. For the infection, a titer of 1 × 104 ml−1 was used. The resulting virus stock was harvested 4 days after infection of 0.45 × 106 cells in a 12-well plate. The averaged TCID50-values are presented as mean values±SD. c Comparison of virus productions depending on the initial infection titer. Experiment design as described in b. Infection titers varied between 1 × 101 and 1 × 106 ml−1. The differences between infection and harvesting titers are shown as amplification factors. High Five cells do not produce a noticeable amount of new infectious viral particles, while Sf9 cells are shown to be highly productive. Different clones of the same cell line can exhibit considerable differences in their virus production capabilities. Data of three independent experiments are presented as mean values ± SD.
