Fig. 1: Comparison between SpyCas9 and LbCas12a for cell-penetrating SV40 NLS-assisted delivery and gene editing in neural cells in vitro and in vivo.
From: Engineering self-deliverable ribonucleoproteins for genome editing in the brain
a Graphic illustrations of two RNP constructs. b Schematic of Cas9 or Cas12a RNP-mediated editing of Ai9 tdTomato NPCs to turn on fluorescent signals. c Quantification of tdTom+ NPCs based on the direct delivery of gene editors. n = 4 for each group, data are presented as mean values with individual data points. d Comparison of the gene editing activities of cell-permeable RNPs based on SpyCas9 and iCas12a in Ai9 mouse brain. e Editing volumes in the striatal tissue based on the injection of different RNP dosages. n = 6 for each group. f Co-expression of tdTomato and NeuN quantified per regions of interest (ROIs), e.g., edited area per hemisphere. n = 4 for the group of iCas12a (250 pmole), n = 5 for the other groups. Statistical analyses (unpaired t test) were performed for (c, e, f) and p values (**p < 0.01, ****p < 0.0001, and n.s. - not significant) were indicated with each set of quantification. Data are presented in box plots for (e, f) where the lower bound of the lower whisker shows the minimum, the lower bound of the box shows the lower quartile, the center of the box shows the median, the upper bound of the box shows the upper quartile and the upper bound of the upper whisker shows the maximum. Images in (a, b, d) were created with biorender.com.
