Fig. 4: Comparison of SV40 NLS-assisted and A22p peptide-assisted delivery of SpyCas9 for gene editing in neural cells in vitro and in vivo.

a Quantification of tdTom⁺ NPCs based on the direct delivery of RNPs to NPCs in vitro. n = 4 for each group, data are presented as mean values with individual data points. Statistical analyses (unpaired t test) were performed, and p-values (****p < 0.0001) were indicated with each set of quantification. b Comparison of the gene editing activities of cell-permeable Cas9 RNPs in Ai9 mouse brain based on the injection of different RNP dosages. c Editing volumes in the striatal tissue. n = 6 for each group. d Co-expression of tdTomato and NeuN quantified per regions of interest (ROIs), e.g., edited area per hemisphere. n = 4 for the group of 2x-A22p3*, n = 5 for the other groups. Statistical analyses (unpaired t test) were performed for (c, d) and p values were indicated with each set of quantification. Data are presented in box plots for (c, d) where the lower bound of the lower whisker shows the minimum, the lower bound of the box shows the lower quartile, the center of the box shows the median, the upper bound of the box shows the upper quartile and the upper bound of the upper whisker shows the maximum. Images in (b) were created with biorender.com.