Fig. 3: Effects of autophagy and mitophagy stimuli on LY-MT contacts.
a Representative Z-projection images of HeLa cells transfected with SPLICSS- P2ALY-MT in untreated condition (CTRL) or treated with CCCP or oligomycinA/ antimycinA (OA/AA) to induces mitophagy or with HBSS or rapamycin to induce starvation. SPLICSS- P2ALY-MT (488 nm), mitochondria and lysosomes were detected by anti-Tom20 or anti-LAMP1 upon excitation at 594 and 405 nm, respectively. b Quantification of short LY-MT contacts in untreated and treated HeLa cells (Mean ± SEM: SPLICSS- P2ALY-MT CTRL 95.9 ± 6.7 n = 30, SPLICSS- P2ALY-MT + CCCP 143.1 ± 6.7 n = 33, SPLICSS- P2ALY-MT + OA/AA 130.9 ± 10.4 n = 27, SPLICSS- P2ALY-MT + HBSS 90.2 ± 9.1 n = 30, SPLICSS- P2ALY-MT + Rapamycin 118.7 ± 8.2 n = 38). c Representative Z-projection images of HeLa cells transfected with SPLICSL- P2ALY-MT in untreated condition (CTRL) or treated with CCCP or oligomycin A/ antimycin A (OA/AA) to induces mitophagy or with HBSS or rapamycin to induce starvation. SPLICSL- P2ALY-MT (488 nm), mitochondria and lysosomes were detected by anti-Tom20 or anti-LAMP1 upon excitation at 594 and 405 nm, respectively. d Quantification of long (SPLICSL- P2ALY-MT CTRL 125.1 ± 9.6 n = 35, SPLICSL- P2ALY-MT + CCCP 150.9 ± 9.6 n = 36, SPLICSL- P2ALY-MT + OA/AA 135.7 ± 10.4 n = 31, SPLICSL- P2ALY-MT + HBSS 143.5 ± 11.4 n = 31, SPLICSL- P2ALY-MT + Rapamycin 128.3 ± 10.6 n = 36) LY-MT contacts in untreated and treated HeLa cells. The SPLICS dots were quantified from the 3D rendering of a complete Z-stack. Scale bar 10 μm. n = cells over 3 independent transfections. (*p ≤ 0.05, **p ≤ 0.01 one-way ANOVA). Source data are provided as a Source Data file.
