Fig. 3: Interaction sites within Eg-SC I + III2 + IV.
From: Euglena’s atypical respiratory chain adapts to the discoidal cristae and flexible metabolism
Individual complexes are colored as labeled throughout this figure (CI: blue; CIII2: green; CIV: magenta). Matrix (a) and IMS (b) view of interaction sites within Eg-SC I + III2 + IV. Individual complexes are shown as colored transparent surfaces. Key subunits are shown as cartoons. c Comparisons of the SC I + III2 + IV or SC I + III2 from E. gracilis, O. aries (PDB 5J4Z) and A. thaliana (PDB 8BPX). Note that since Eg- and ovine respirasomes are shown side by side, positional comparisons of their CIII2 and CIV are visualized by color-coded lines (green: Eg-; red: ovine) marking translational and rotational positions of respective complexes. Translational distances and rotational angles are labeled. d Comparison of CI-CIII2 angle in E. gracilis, O. aries and T. thermophila (PDB 7TGH), viewed from alongside the membrane plane. TMHs of ND5 and COB are shown in colored cartoons indicating the membrane curvatures, NDUEG7 is shown in solid surface. CG MD simulations of Eg-SC I + III2 + IV (e) and Tt-SC I + III2 (f) in lipid bilayers demonstrate that they induce negative and positive membrane curvatures respectively. Individual complexes are colored as in Figs. 1, 2. The CG lipid phosphate head groups are shown as yellow spheres.
