Fig. 8: Generation of SPW-Rs by optical stimulation of CA1 PYR is abolished by pharmacological activation of CA1 CB1Rs.

a Illustration of the experimental setup. A head-fixed AAV-CamKII-ChR2 mouse is mounted on the wheel. A virtual reality environment is presented on the screen to instigate running, and the virtual position is recorded. Local infusion, optical stimulation, and neural recording are achieved using the T-DOpE probe. b Power spectrogram of CP-55,940 infusion session on high frequency bins (100–300 Hz). Following 30 min of baseline, CA1 PYR are optical stimulated (n = 400 pulses of 150 ms at low, medium, and high power) over 40 min. CP-55,940 (mg kg⁻¹; 200 nL; 1 nLs⁻¹) is locally infused at the 80-minute mark. After the cells recover from the infusion, optical stimulation is repeated. c Representative response to low, medium, and high optical stimulations during the baseline and after CP-55,940 infusion. d Average neural response to the high optical stimulation during the baseline and after CP-55,940 infusion. Data are presented as mean ± SD. e Average Wavelet Transform of the activity in baseline and after drug infusion. f Spectrum of baseline and for CP-55,940 infusion during high pulses. g Spectral difference between the baseline and after CP-55,940 infusion for the optical pulses. h The averaged normalized spectrum (animal number = 4). Data are presented as mean ± SD. i The averaged spectral difference between the baseline and after CP-55,940 infusion for high optical pulses (animal number = 4). Data are presented as mean ± SD. j Normalized ripple rate during baseline and during optical stimulation. After the infusion, normalized ripple rate 30 min before and during optical stimulation. (two-sided Wilcoxon Signed-rank test, *****p ≤ 0.00001, (Baseline vs. CP-55,940, p = 1.8162e-5, animal number = 4), (Optical Stim vs. Optical Stim after CP-55,940, p = 8.2773e-6, animal number = 4)). Data are presented as mean ± SEM.