Fig. 1: All B cells are recipients of a Notch signal, which gradually increases during B cell development and is highest in Marginal Zone B cells.

a The graph compiles the Median Fluorescence Intensity (MFI) of H2B-Venus in different B cell populations from CBF:H2B-Venus mice in the bone marrow (BM) and the spleen. The B cell populations were gated as shown in Supplementary Fig. 1a, b. For Marginal Zone B (MZB) cells the MFI for the H2B-Venus^high peak, as gated in (b) is depicted. The bar graph shows mean values, standard deviations (SD), and individual data points from n = 4 mice (1 male (m), 3 females (f)). Data were logarithmized before analysis (***p = 0.0005, ****p < 0.0001), ordinary one-way ANOVA, Tukey´s multiple comparison test. b The histogram overlay shows the H2B-Venus expression in the indicated gated cell populations (red: pro/early preB; blue: Follicular B (FoB); pink: MZB). c The graphs compile the relative expression levels of the known Notch-target genes Dtx1 (Deltex1), Hes1, and Cr2 (Complement receptor 2 = CD21) in FoB cells from N2^fl/fl//CD19-Cre (N2KO) (blue) n = 5 mice, and controls (ctrl: CD19-Cre) (red) n = 7 mice. The scatter plots show individual data points with means. Unpaired two-tailed t-tests were performed (***p = 0.0005, ****p < 0.0001). The data are extracted from an Illumina Microarray-based gene expression profiling described in the thesis of S. Ehrenberg (available from https://doi.org/10.5282/edoc.19322)⁶⁴ and are available at ⁶¹. d MFIs of H2B-Venus and Hes1 in FoB (gray), pre-MZB (blue), and MZB (red) cells, which were gated as shown in the contour plot (left). Representative histogram overlays for H2B-Venus are shown in the middle. MFIs for H2B-Venus in MZB cells were only determined in the gated population of the histogram (H2B-Venus^high). After log transformation data were analyzed by a repeated measures (RM) one-way ANOVA, Tukey´s multiple comparison test n = 8 mice (4 m, 4 f) for H2B-Venus: ****p < 0.0001, **p = 0.0067; n = 7 mice (5 m, 2 f) for Hes1: ****p < 0.0001. Source data from (a), (c), and (d) are provided as a Source Data file.