Fig. 8: Mathematical modeling of CAR expression dynamics in B cells during an ongoing TD-immune response. | Nature Communications

Fig. 8: Mathematical modeling of CAR expression dynamics in B cells during an ongoing TD-immune response.

From: Notch2 controls developmental fate choices between germinal center and marginal zone B cells upon immunization

Fig. 8

a, b Correlation plots of CAR expression kinetics in Follicular (FoB), Marginal Zone B (MZB), and Germinal Center B (GCB) cell subsets. We compare the changes in the logarithmic (ln) counts of CAR+ B cells in each subset and show their correlation coefficient (r) on each plot. The sequential gating strategy to retrieve the frequencies of each cell population is depicted in Supplementary Fig. 11. The data are grouped in different bins based on the time of observation following TD-immunization, indicated by different colors in the plots: 1 week (red) (n = 8; 5 males (m), 3 females (f)), 2 weeks (turquoise) (n = 12, 7 m, 5 f), 3 weeks (green) (n = 18; 8 m, 10 f), 4 weeks (dark blue) (n = 14; 7 m, 7 f), control/CAR mice after TD-immunization. c–e Schematics of the general forms of the branched, linear, and null models of CAR+ MZB cell generation upon TD-antigen-mediated B cell activation. In-depth details about model specifics are given in the Materials and Methods. Source data are provided as a Source Data file (see sheets modeling data).

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