Fig. 2: Endogenous RutR-FLAG protein levels are constant during E. coli growth. | Nature Communications

Fig. 2: Endogenous RutR-FLAG protein levels are constant during E. coli growth.

From: Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism

Fig. 2

a Genomic insertion of a FLAG-tag encoding region downstream of rutR or genomic deletion of rutR does not affect growth behavior of E. coli BW30270 and E. coli U65 in complex medium (LB). The experiment was performed in duplicates and the graph depicts the means (solid lines) ± standard deviations (dashed lines) of both recorded replicates (n = 2). Source data are provided as Source Data file. b Endogenous RutR-FLAG protein level is constant during E. coli BW30270 growth. Left panel: immunoblotting (IB) of RutR-FLAG with anti-FLAG antibody (IB: FLAG). 2,2,2-trichloroethanol staining (TCE) served as loading control. Right panel: Quantitative analysis of the RutR-FLAG expression level was performed by analysis of immunoblottings using ImageJ software. One exemplary out of four replicates is shown and bars depict means ± standard deviations. Significance was tested by t-tests and no significant differences were detected by application of a significance level of p < 0.05 (n = 4). Source data are provided as Source Data file.

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