Fig. 5: AT and K absorption spectra, excitation spectra and AT photoreaction scheme. | Nature Communications

Fig. 5: AT and K absorption spectra, excitation spectra and AT photoreaction scheme.

From: Retinal photoisomerization versus counterion protonation in light and dark-adapted bacteriorhodopsin and its primary photoproduct

Fig. 5

A Absorption spectra of all-trans (AT) and K intermediate along with excitation pulse spectra. B A representative scheme of AT photoreaction in light-adapted bacteriorhodopsin (LA-BR) and in the presence and absence of K. It also shows the photoreaction of K and isolation of pure K photochemistry. Exp..1 represents the AT-BR’s photochemistry. Exp..2 uses two excitation pulses, a few ps after the actinic excitation of AT-BR a mixture of AT-BR and K intermediate was produced. TA measurement after 60 ps of the actinic excitation records the photochemistry of the AT-BR and K. Subtracting the correct weighted contribution of AT-BR’s data from Exp..2 gives the photochemistry of K. Source data are provided as a Source Data file.

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