Table 3 Antibodies overview
From: Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation
Antigen | Supplier (cat#) | Clone | Dilution | Antigen retrieval |
|---|---|---|---|---|
C1qB | Abcam (ab92508) | EPR2981 | 1:100a | Tris EDTA buffer pH9 |
C3d | Dako (A0063) | Polyclonal | 1:300 | Citrate buffer pH6 |
C5b9 | Dako (M077701-8) | aE11 | 1:100 | Citrate buffer pH6 |
CD138 | BioRad (MCA2459T) | B-A38 | 1:250 | Citrate buffer pH6 |
CD20 | Dako (M0755) | L26 | 1:100 | Citrate buffer pH6 |
CD3 | Dako (A0452) | Polyclonal | 1:100 | Citrate buffer pH6 |
CD38 | Atlas antibodies (HPA022132) | Polyclonal | 1:3000 | Citrate buffer pH6 |
CD4 | Dako (M7310) | 4B12 | 1:100 | Tris EDTA buffer pH9 |
CD8 | BD Biosciences (641400) | SK1 | 1:500 | Citrate buffer pH6 |
DAGLB | Atlas Prestige (HPA069377) | Polyclonal | 1:50a | PBS pH7.6 |
E06 | Avanti (330002 S) | T15 | 1:100 | PBS pH7.6 |
FABP5 | RabMab (ab255276) | EPR22552-64 | 1:2000 | Tris EDTA buffer pH9 |
HLA-DR-DP-DQ | Dako (M0775) | CR3/43 | 1:100 | Citrate buffer pH6 |
IAH1 | Invitrogen (PA5-65270) | Polyclonal | 1:50a | PBS pH7.6 |
Iba1 | Wako (019-19741) | Polyclonal | 1:500 | Citrate buffer pH6 |
IGG | Abcam (ab218427) | Polyclonal | 1:100 | Citrate buffer pH6 |
LAMP1 | Abcam (ab24170) | Polyclonal | 1:200 | Citrate buffer pH6 |
MBP | Sigma (AB980) | Polyclonal | 1:200 | Citrate buffer pH6 |
PCNA | Santa Cruz (sc-25280) | PC10 | 1:1000 | Citrate buffer pH6 |
PLP | Secotec (MCA839G) | plpc1 | 1:3000 | Citrate buffer pH6 |
SMI312 | Eurogentec (SMI-312R) | SMI-312R | 1:6000 | Citrate buffer pH6 |
STARD13 | Invitrogen (PA5-63622) | Polyclonal | 1:300a | PBS pH7.6 |
Tomm20 | RabMab (ab186735) | EPR15581-54 | 1:100a | Citrate buffer pH6 |
VWF | Atlas antibodies (AMAb90928) | CL1950 | 1:500a | Citraconic anhydride pH6 |
