Fig. 5: Feedback circuits in the hormonal cell–cell communication network.
From: An organism-wide atlas of hormonal signaling based on the mouse lemur single-cell transcriptome
a Definition of two-node feedback circuits. b Comparison of the number of 2-node feedback circuits identified in the hormonal cell–cell communication network (red dashed line) and that of permuted networks (n = 1000), using one-tailed one-sample t-test. Permutation preserved node outdegree and indegree. c–l Examples of two-node feedback circuits identified in the network, focusing on the endocrine cell types. Solid black arrows indicate known regulation, dashed orange arrows indicate predicted regulation with partial literature support, and dotted arrows indicate predicted regulation without earlier knowledge that are potentially biologically relevant (red) or insignificant (gray). A leg is considered biologically insignificant if the connected hormone-producing cell type is unlikely to be the major source of the hormone (cells had lower expression levels than the canonical major source cells) and if the signaling is not local. Parentheses are used to group multiple genes that are expressed in the relevant cell type that function either together (&) or independently (/).
