Fig. 3: Intermolecular interfaces between 70SL and 70SQ. | Nature Communications

Fig. 3: Intermolecular interfaces between 70SL and 70SQ.

From: Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM

Fig. 3

a Overview of the cryo-EM structure of the disome complex containing 70SL and 70SQ in closed non-rotated PRE states. Shown is a composite map generated from locally refined 70SL, 70SQ, and interface maps. Shown are 50SL (blue), 30SL (yellow), 50SQ (lavender), 30SQ (peach), bL9L (turquoise), EF-G (red), A-tRNAs (light violet), P-tRNAs (green), E-tRNAs (orange), and bS1Q (pink). Map corresponding to bS1Q is segmented and shown at lower threshold. Close-ups of the five interfaces between 70SL and 70SQ (shown is the interface map). Local resolution ranges of map regions shown in close-ups: (b) 4–6.3 Å, (c) 3.0–3.6 Å, (d) 3.3–3.8 Å, (e) 4–4.4 Å, (f) 2.7–3.5 Å. g Overlay of bL9L bound to 30SQ and EF-G (10 Å map simulated from an EF-G•GTP model (PDB: 7N2V21).

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