Fig. 5: Binding domain of mAb-9D5 on the NP. | Nature Communications

Fig. 5: Binding domain of mAb-9D5 on the NP.

From: Nucleocapsid protein-specific monoclonal antibodies protect mice against Crimean-Congo hemorrhagic fever virus

Fig. 5

A Structural representation of the NP monomer. The dashed box indicates the magnified NP region depicting the mAb-9D5 binding region identified by LC-MS. B Epitope amino acid comparisons between diverse strains of CCHFV and related nairoviruses IbAr 10200 (accession #MH483987.1), Afg09 (accession #HM452305.1), Kosova Hoti (accession #JN173797.1), Oman (accession #DQ211645.1), Semunya (accession #DQ076413), Senegal (accession #DQ211640), Aigai virus (accession #NC_078226), Hazara virus (accession #NC_038711) and Erve virus (accession #JF911699). C BLI binding kinetics of mAb-9D5 hybridoma with nairovirus NPs. BLI SA biosensors were loaded with NP (antigen) at concentration of 500 nM. Anti-NP mAb was evaluated in triplicate at concentrations of 700, 350, and 100 nM. NB not binding. Source data is provided.

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