Fig. 2: Inhibition of mitochondrial serine catabolism disrupts de novo purine synthesis and glycolysis in CML cells. | Nature Communications

Fig. 2: Inhibition of mitochondrial serine catabolism disrupts de novo purine synthesis and glycolysis in CML cells.

From: Inhibition of mitochondrial folate metabolism drives differentiation through mTORC1 mediated purine sensing

Fig. 2

LC-MS measurement of intracellular glycine (a), ribose-5-phosphate (R5P) (b), purine biosynthetic intermediate and nucleotides (c) and glycolysis related metabolites (d) in K562 cells treated with 2.5 μM SHIN1 with or without the addition of 1 mM formate for 24 h (n = 4 independent cultures). e Glucose uptake and lactate secretion in K562 cells in response to treatment as explained in (a–d) (n = 5 independent cultures). Representative extracellular acidification rate (ECAR) profile (f), relative glycolysis and glycolytic capacity (g) as measured by the Seahorse XF analyser of K562 cells treated as in (a–e) (n = 4 independent cultures). Data are shown as the mean ± s.e.m. P-values are derived from a repeated measure one-way ANOVA with Dunnett’s multiple comparisons test (a–e) or ordinary one-way ANOVA with Dunnett’s multiple comparisons test (g). Source data are provided as a Source Data file.

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