Fig. 5: Imatinib maintains serine contribution to glutathione. | Nature Communications

Fig. 5: Imatinib maintains serine contribution to glutathione.

From: Inhibition of mitochondrial folate metabolism drives differentiation through mTORC1 mediated purine sensing

Fig. 5

Fractional labelling of serine, glycine (a), GSH, GSSG (b), and purine nucleotides (AMP, ATP, GMP, GTP) (c) from K562 cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13C315N1-serine. (n = 4 independent wells from individual experiment). Schematics for serine and glycine structures were created with Biorender.com (Agreement numbers: EZ26CFMM6G and WI26CFWJC3 respectively). Fractional labelling of serine, glycine (d) and GSH, GSSG (e) from CML CD34+ cells treated for 24 h with 2 μM imatinib (IM), 2.5 μM SHIN1, or a combination of both in medium containing 140 μM 13C315N1-serine (n = 4 patient samples). Fractional labelling represents fraction of the sum of all labelled isotopologues for each metabolite. Schematic for patient cells created with Biorender.com (Agreement number SH26CFO8OE). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Dunnett’s multiple comparisons test (d, e). Source data are provided as a Source Data file.

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