Fig. 4: Global reduction of H3K27me3 enables unrestricted cell fate of the spontaneous qESCs.

a Left, heatmaps of H3K27me3 CUT&RUN signals from biological duplicates. The number of differential peaks was shown on top. Right, line plots showing H3K27me3 peak signals from the left. b Left, immunofluorescence of H3K27me3 (green) and nuclei (DAPI blue) in high and low ΔΨ_m ESCs. Scale bar, 10 µm. Right, quantified H3K27me3 fluorescence signals of polycomb bodies (foci) relative to DAPI. Violin plots show the median, 1st and 3rd quartiles. Data from three biological replicates were presented. P values were determined by a two-tailed, nonparametric Mann-Whitney test. n number of foci. c GO analysis for H3K27me3 peaks that were decreased in the low ΔΨ_m qESCs. “Count” denotes the number of genes found in each GO term. d Top, line plots showing changes of H3K27me3, H3K4me3, and ATAC-seq signals at 662 MERVL elements in low and high ΔΨ_m ESCs. Bottom, violin box plots representing the peak values of the top plots show the median, 1st, 3rd quartiles, lower adjacent value, upper adjacent value, and outside points. P values were determined by a nonparametric Mann-Whitney test. e Levels of intracellular SAM in naive ESCs treated with DMSO or CCCP. Data were presented as mean ± SEM from four biological replicates. a.u. arbitrary unit. P values were determined by the two-sided Student’s t test. f Left, immunofluorescence of H3K27me3 (green) and nuclei (DAPI blue) at indicated conditions. Scale bar, 10 µm. Right, quantification of the left. Violin plots denote quantified H3K27me3 fluorescence signals of foci relative to DAPI. Data were from three biological replicates. P values were determined by a two-tailed, nonparametric Mann-Whitney test. n number of foci. Source data are provided as a Source Data file.