Fig. 8: Quiescence preserves innate unrestricted cell fate in naive ESCs.

a Top, scheme for generating new naive ESC lines from the low ΔΨ_m single qESC. Bottom, TMRM staining showing the similarity of ΔΨ_m heterogeneity between parental cells and three newly established naive ESC lines. b RT-qPCR analysis for genes related to 2C (left) and pluripotency (right) in high and low ΔΨ_m ESCs isolated from three single cell derived naive ESC lines. Relative mRNA expression was normalized against that in the high ΔΨ_m ESCs, which was arbitrarily set 1. Average values were presented from three single cell derived naive ESC lines. c Left, immunofluorescence of OCT4 (red), CDX2 (green) and nuclei (DAPI blue) for TSC induction at day 7 from three single cell derived naive ESC lines. Scale bar, 200 µm. Representative images from three independent experiments for each cell lines were shown. d Quantification of CDX2⁺/OCT4⁻ cells for data shown in (c). Average values were presented from three single cell derived naive ESC lines. Source data are provided as a Source Data file.